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Cell therapy (also called cellular therapy or cytotherapy) is therapy in which cellular material is injected, grafted or implanted into a patient; this generally means intact, living cells. For example, T cells capable of fighting cancercells via cell-mediated immunity may be injected in the course of immunotherapy.
Chimeric antigen receptor T cells (also known as CAR T cells) are T cells that have been genetically engineered to produce an artificial T-cell receptor. CAR-T cell therapy uses T cells engineered with CARs for cancer therapy. The premise of CAR-T immunotherapy is to modify T cells to recognize cancer cells in order to more effectively target and destroy them. Scientists harvest T cells from people, genetically alter them, then infuse the resulting CAR-T cells into patients to attack their tumors. CAR-T cells can be either derived from T cells in a patient's own blood (autologous) or derived from the T cells of another healthy donor (allogenic). Once isolated from a person, these T cells are genetically engineered to express a specific CAR, which programs them to target an antigen that is present on the surface of tumors. For safety, CAR-T cells are engineered to be specific to an antigen expressed on a tumor that is not expressed on healthy cells.
ADAM-MC series can be used as a device for monitoring and QC of the cell numbers and viability in the process of manufacturing cells (CAR-T cells, stem cells, etc.) for Cell Therapy. In addition, it is possible to use ADAM-MC2 depending on the cell types (Whole blood cell, PBMCs, etc.) that needs to be monitored during the manufacturing of cell therapy products.
More than 100 cell therapy research institutes and companies around the world use ADAM-MC and ADAM-MC2.
Technology and patent for counting cells
ADAM-MC2 counts cells based on a fluorescent microscopy technology. The ADAM-MC2 utilizes sensitive fluorescence dye staining, LED optics and CMOS detection technologies to make the cell analysis more accurate and reliable. To count cells using ADAM-MC2, the cells are mixed with a Propidium Iodide (PI) stain and directly pipetted on to a disposable plastic chip. The chip is then loaded onto a precision stage. ADAM-MC2 system is automatically focused onto the chip and cells that have been stained are recorded by a sensitive CMOS camera. The image results are automatically processed generating the cell count which is displayed on the front of the instrument. Simple. Fast. Accurate. Reliable.
Principle of Viability Measurement (PI-staining Method)
After the samples are stained with fluorescent dye, propium iodide, which intercalates DNA to stain the nucleus of target cells, fluorescent images are taken automatically. The obtained images are processed by image analysis software integrated inside the system.
Accuracy, Repeatability, and Comparison of cell viability
Comparison of cell viabilities with ADAM-MC2, flow cytometry, and manual counting. SH-SY5Y, Jurkat, HeLa dells were treated with 100, 300 μM H2O2 for 3 hours, then analyzed by ADAM-MC2, flow cytometry, and manual counting.