메뉴 바로가기

본문으로 바로가기

Home / 제품소개 / 상세페이지

Fluorescence CD34 Counter

ADAMⅡ is a bench top fluorescent CD34 counter that can be used in hematopoietic stem cell transplant for stem cell therapy.

KEY FEATURES & BENEFITS
- 15 minutes for counting CD34
- Easy to use
- Accurate and reliable result
- 4 channel (Bright, PerCP, PE, FITC)
- State-of-the-arts optics and image analysis software




ADAMⅡ, Fluorescence CD34 Counter 
ADAMⅡ is a bench top fluorescent CD34 counter that can be used in hematopoietic stem cell transplant for stem cell therapy.




ADVANTAGE #1

Revolutionary new way to count CD34!

ADAMⅡ is an image-based fluorescence cell counter, which captures and analyzes multiple images.
It has 4-channel (Bright, Yellow (PE), Red (PerCP) and Green (FITC)) light sources.

Following specimens can be analyzed: normal and mobilized peripheral blood (PB/MPB),
as well as fresh hematopoietic progenitor cell-apheresis (HPC-A) collection.

ADAMⅡ measures the percentage of viable CD34 positive cells from total leukocytes and absolute number of CD34 positive cells (cells/μL).





ADVANTAGE #2

Simple and easy to use

Add the sample and reagent to an empty tube, then add the lysis buffer.
Load the stained sample onto the slide and insert into the ADAMⅡ instrument to get the result.

Up to 7 results will be generated and displayed : (1) Total CD45 (cells/μL) (2) Viable CD45 cells/μL (3) Total CD34 (%, cells/μL)
(4) Viable CD34 (%, cells/μL) (5) Viable CD34 of Viable CD45 (%) (6) CD45 Viability (%) (7) CD34 Viability (%).


ADAMⅡ provides an accurate and precise results with rapid turnaround and minimal hands-on time by the operator.
The high degree of precision of the system is due in part to the simplifying sample preparation steps and
eliminating washing and reconstituting steps, which may cause cell loss and human error.
In addition, results are reported using the test specific software, limiting interpretation steps by the user.










ADVANTAGE #3

Substantially equivalent to flow cytometry

Mobilized PB and PBSC samples were used to compare with ADAMⅡ and flow cytometry.
Refer to the comparison data, linear regression analysis revealed a close correlation
between the data for the absolute number of CD34+ and its cell fractions(%) obtained with the ADAMⅡ and the flow cytometry.

The correlation for CD34 cell counts between the ADAMⅡ and the flow cytometry
(A) Absolute number of CD34+ cells, (B) Percentage of CD34 +cells
(A: Total CD34
(Cells/μL), B: Total CD34 (%))







 

• ADAMⅡ VS Flow cytometey (Mobilized PB)
The correlation for CD34 cell counts between the ADAMⅡ and the flow cytometry
(A) Absolute number of CD34+ cells, (B) Percentage of CD34 +cells
(A : Viable CD34 (Cells/μL) B : Viable CD34 (%))







  • Product name / Cat. No.

    ADAMⅡ / ADAMⅡ

  • Camera

    High-sensitivity monochrome CCD

  • Optical

    Bright, Yellow (PE), Red (PerCP) and Green (FITC)

  • Weight

    19.3 kg

  • Size (LxWxH)

    300 x 420 x 370 mm

  • Lens

    Olympus lens (10X) / 35∮tube lens

  • Specimen

    HPC-A, Mobilized PB, Control

  • Filter

    PerCP: BLP01-635R (650 long pass)

  • Total frame / Total Volume

    136 frame / 7.854 uL

  • PE: Excitation 525/50, Emission 572/28-25

  • Reading range / Chamber Volume

    28 x 8 x 0.1 mm / 25 uL

  • FITC: Excitation 466/40, Emission 550/50

  • Stage

    Automated X-Y-Z stage