ADAMⅡ is a bench top fluorescent CD34 counter that can be used in hematopoietic stem cell transplant for stem cell therapy.
Revolutionary new way to count CD34!
ADAMⅡ is an image-based fluorescence cell counter, which captures and analyzes multiple images. It has 4-channel (Bright, Yellow (PE), Red (PerCP) and Green (FITC)) light sources.
Following specimens can be analyzed: normal and mobilized peripheral blood (PB/MPB), as well as fresh hematopoietic progenitor cell-apheresis (HPC-A) collection.
ADAMⅡ measures the percentage of viable CD34 positive cells from total leukocytes and absolute number of CD34 positive cells (cells/μL).
Simple and easy to use
Add the sample and reagent to an empty tube, then add the lysis buffer. Load the stained sample onto the slide and insert into the ADAMⅡ instrument to get the result.
Up to 7 results will be generated and displayed : (1) Total CD45 (cells/μL) (2) Viable CD45 cells/μL (3) Total CD34 (%, cells/μL) (4) Viable CD34 (%, cells/μL) (5) Viable CD34 of Viable CD45 (%) (6) CD45 Viability (%) (7) CD34 Viability (%).
ADAMⅡ provides an accurate and precise results with rapid turnaround and minimal hands-on time by the operator. The high degree of precision of the system is due in part to the simplifying sample preparation steps and eliminating washing and reconstituting steps, which may cause cell loss and human error. In addition, results are reported using the test specific software, limiting interpretation steps by the user.
Substantially equivalent to flow cytometry
Mobilized PB and PBSC samples were used to compare with ADAMⅡ and flow cytometry. Refer to the comparison data, linear regression analysis revealed a close correlation between the data for the absolute number of CD34+ and its cell fractions(%) obtained with the ADAMⅡ and the flow cytometry.
The correlation for CD34 cell counts between the ADAMⅡ and the flow cytometry (A) Absolute number of CD34+ cells, (B) Percentage of CD34 +cells (A: Total CD34 (Cells/μL), B: Total CD34 (%))
• ADAMⅡ VS Flow cytometey (PBSC) The correlation for CD34 cell counts between the ADAMⅡ and the flow cytometry (A) Absolute number of CD34+ cells, (B) Percentage of CD34 +cells(A : Viable CD34 (Cells/μL) B : Viable CD34 (%))